Nationales Referenzzentrum (NRZ) für Systemische Mykosen Institut für Medizinische Mikrobiologie Leitung: Prof. Dr. U. Groß

Aspergillose - Diagnostik

Weig, M., M. Frosch, A. Haas, U. Groß, and J. Heesemann. 2001. Use of recombinant mitogillin for improved serodiagnosis of Aspergillus fumigatus associated diseases. J. Clin. Microbiol., in press.

Andreas, S., C. Heindl, K. Wattky, C. Möller, and R. Rüchel. 2000. Diagnosis of pulmonary aspergillosis using optical brighteners. Eur. Respir. J. 15: 407-411

Kambouris, M. E., U. Reichard, N. J. Legakis, and A. Velegraki. 1999. Sequences from the aspergillopepsin PEP gene of Aspergillus fumigatus: evidence on their use in selective PCR identification of Aspergillus species in infected clinical samples. FEMS Immunol. Med. Microbiol. 25: 255-264

Rüchel, R., and M. Schaffrinski. 1999.Versatile fluorescent staining of fungi in clinical specimens using the optical brightener Blankophor-P. Clin. Microbiol. 37: 2694-2696

Rüchel, R., M. Schaffrinski, K. R. Seshan, and G. T. Cole. 1999. Vital staining of fungal elements in deep seated mycotic lesions during experimental murine mycoses using the parenterally applied optical brightener Blankophor. Med. Mycol. 38: 231-237

Weig, M., Rath, P.-M., and U. Groß. 1997. Restrictocin - Welche Bedeutung hat es für Pathogenese und Diagnostik der invasiven Aspergillose? Hyg. Mikrobiol. 2/97: 12-17.
 

Aspergillose - Klinik und Therapie

Lorf, T., F. Braun, R. Rüchel, A. Müller,  B. Sattler, and B. Ringe. 1999. Systemic mycoses during prophylactical use of liposomal amphotericin B (Ambisome ®) after liver transplantation. Mycoses 42: 47-53

Braun, F., R. Rüchel, R. Lorf, R. Canelo, A. Müller, B. Sattler, and B. Ringe. 1998. Is liposomal amphotericin B (Ambisome) an effective prophylaxis of mycotic  infections after liver transplantation? Transpl. Proceedings, 30: 1481-1483

Kern, W., J. Braess, H. Bertz, C. Pott, E. Schleyer, J. Finke, R. Rüchel, W. Hiddemann. 1998. Chronic systemic aspergillosis in a patient with acute myeloid leukemia. Ann. Hematol. 76: 175-177

Singer, S., D. Singer, R. Rüchel, H. Mergeryan, U Schmidt, K. Harms. 1998. Gehäuftes Auftreten systemischer Aspergillosen auf einer neonatologischen Intensivstation. Mycoses 41: 223-227
 

Aspergillose - Forschung

Reichard, U., G. T. Cole, T. W. Hill, R. Rüchel, and M. Monod. 2000. Molecular characterization and influence on fungal development of ALP2, a novel serine proteinase from Aspergillus fumigatus. Int. J. Med. Microbiol. 290:  549-558

Reichard, U., G. T. Cole, R. Rüchel, and M. Monod. 2000. Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus. Int. J. Med. Microbiol. 290: 85-96

Reichard, U., M. Monod, F. Odds, and R. Rüchel. 1997. Virulence of an aspergillopepsin-deficient mutant of Aspergillus fumigatus and evidence for another aspartic proteinase linked to the fungal cell wall. J. Med. Vet. Mycol. 35: 189-196

Reichard, U., M. Monod, and R. Rüchel. 1996. Expression pattern of aspartic proteinase antigens in aspergilli. Mycoses 39: 99-101

Reichard, U., M. Monod, and R. Rüchel. 1995. Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus. FEMS Microbiol. Lett. 130: 69-74

Reichard, U., H. Eiffert, and R. Rüchel. 1994. Purification and characterization of an extracellular aspartic proteinase from Aspergillus fumigatus. J. Med. Vet. Mycol. 32: 427-436

Reichard, U., S. Büttner, H. Eiffert, F. Staib, and R. Rüchel. 1990. Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue. J. Med. Microbiol. 33: 243-251
 

Candidose - Diagnostik

Reichard, U., S. Margraf, B. Hube, and R. Rüchel. 1997. A method for recovery of Candida albicans DNA from larger blood samples and its detection by polymerase chain reaction on proteinase genes. Mycoses 40: 249-253

Borg-von Zepelin, M. and V. Grüneß. 1993. Characterization of two monoclonal antibodies against secretory proteinase of Candida tropicalis DSM 4238. J. Med. Vet. Mycol. 31: 1-15.
 

Candidose- Klinik und Therapie

Müller, F. M. C., M. Weig, J. Peter, and T. J. Walsh. 2000. Azole cross-resistance to ketoconazole, fluconazole, itraconazole and voriconazole in clinical Candida albicans isolates from HIV-infected children with oropharyngeal candidosis. J. Antimicrob. Chemother. 46: 338-341.

Weig, M., E. Werner, M. Frosch, and H. Kasper. 1999. Limited effect of refined carbohydrate dietary supplementation on colonization of the gastrointestinal tract of healthy subjects by Candida albicans. Am. J. Clin. Nutr. 69: 11-70-1173.

Hermann, C., J. Hermann, U. Munzel, and R. Rüchel. 1999. Bacterial flora accompanying Candida yeasts in clinical specimens. Mycoses, 42: 619-627

Weig, M. 1996. Pilzbesiedelung des Gastrointestinaltraktes: Einfluß von Ernährungsfaktoren, Aktuelle Ernährungsmedizin 21: 118-119.
 

Candidose - Forschung

Borg-von Zepelin, M., T. Niederhaus, U. Groß, M. Seibold, M. Monod, and K. Tintelnot. 2002. Adherence of different Candida dubliniensis strains in the presence of fluconazole. AIDS, in press.

Zaugg, C., M. Borg-von Zepelin, U. Reichard, D. Sanglard, and M. Monod. 2001. Secreted Aspartic Proteinase Family of Candida tropicalis. Infect. Immun. 69: 405-412

Beggah, A., B. Lechenne, U. Reichard, S. Foundling, and M. Monod. 2000. Intra- and intermolecular events direct the propeptide-mediated maturation of the Candida albicans secreted aspartic proteinase Sap1p. Microbiology 146: 2765-2773

Schaller, M.,  H.C. Korting, B. Hube, W. Schäfer, M. Borg-von Zepelin, E. Thoma-Grebe, and M. Ollert. 1999. Evidence for In vivo expression of Candida albicans secreted aspartyl proteinases in oropharyngeal candidiasis by immunoelectron microscopy and rt-pcr. J. Invest. Dermatol. 112: 383-386.

Monod, M., M. Borg-von Zepelin, A. Telenti and D. Sanglard. 1999. The inhibition of Candida albicans secreted aspartic proteases by three different HIV protease inhibitors. Dermatology 198: 412-414.

Gruber, A., C. Speth, E. Lukasser-Vogl, R. Zangerle, M. Borg-von Zepelin, M. P. Dierich, and R. Würzner. 1999. Human immunodefiency virus type 1 protease inhibitor attenuates Candida albicans properties in vitro. Immunopharmacol.41: 227-234 .

Borg-von Zepelin, M., I. Meyer, R. Thomssen, D. Sanglard, A. Telenti, and M. Monod. 1999. HIV protease inhibitors reduce cell adherence of Candida albicans strains by inhibition of yeast secreted aspartic proteases. J. Invest. Dermat. 113: 747-751.

Gruber, A.,  E. Lukasser-Vogl, M. Borg-von Zepelin, M. P. Dierich, and R. Würzner. 1998. Human immunodefiency virus type 1 gp160 and gp41 binding to Candida albicans selectively enhances Candida virulence in vitro. J. Infect. Dis. 177: 1057-1063.

Borg-von Zepelin, M.,  S. Beggah, K. Boggian, D. Sanglard, and M. Monod. 1998. The expression of the secreted aspartyl proteinases Sap4 to Sap6 from Candida albicans in murine macrophages. Mol. Microbiol. 28: 543-554.

Borg-von Zepelin, M., J. Eucker, and R. Rüchel. 1997. Beteiligung sekretorischer Candida-Proteinasen an der Adhärenz von C. tropicalis Blastoconidien im Zellkultur-Modell. Mycoses 40:, (Suppl. I):  64-72.

Ollert, M. W., C. Wende, M. Görlich, C. McMullen-Vogel, M. Borg-von Zepelin, C.W. Vogel, and H. C. Korting. 1995. Increased Expression of Candida albicans Secretory Proteinase, a Putative Virulence Factor, in Isolates from Human Immunodefiency Virus-Positive Patients. J. Clin. Microbiol. 33: 2543-2549.

Borg-von Zepelin, M., and T. Wagner. 1995. Fluorescence assay for the detection of adherent Candida yeasts to target cells in microtest plates. Mycoses, 38: 339-347.

Wagner, T., M. Borg- v. Zepelin, and R. Rüchel. 1995. pH-dependent denaturation of extracellular aspartic proteinases from Candida species. Journal of Medical & Veterinary Mycology 33: 275-278

Trinel, P. A.,  M. Borg-von Zepelin, G. Lepage, T. Jouault, D. Mackenzie, and D. Poulain. 1993.  Isolation and Preliminary Characterization of the 14- to 18-Kilodalton Candida albicans Antigen as a Phospholipomannan Containing ß-1,2-Linked Oligomannosides. Infect. Immun. 61: 4398 – 4405.

Borg-von Zepelin, M., and P. Schuff-Werner 1992. Chemiluminescence of polymorphonuclear granulocytes in the presence of selected Candida species.
Mycoses 35: 121-129 .

Borg, M., and R. Rüchel 1990. Demonstration of fungal proteinase during phagocytosis of Candida albicans and Candida tropicalis. J. Med. Vet. Mycol. 28: 3-14.

Borg, M., D. Watters, B. Reich, and R. Rüchel. 1988. Production and Characterization of Monoclonal Antibodies against Secretory Proteinase of Candida albicans CBS 2730. Zentralbl. Bakt., Mikrobiol. Hyg. Orig A, 268: 62 - 73

Borg, M., and R. Rüchel 1988. Expression of Extracellular Acid Proteinase by Proteolytic Candida spp. during Experimental Infection of Oral Mucosa. Infect. Immun. 56: 626 – 631.

Rüchel, R.,  B. Böning, and M. Borg.1986. Characterization of a Secretory Proteinase of Candida parapsilosis and Evidence for the Absence of the Enzyme during Infection in Vitro. Infect. Immun. 53: 411- 419.

Borg, M., D. Kirk,  H. Baumgarten, and R. Rüchel. 1984. A colorimetric assay for the assessment of cytotoxicity of yeasts. Saubouraudia: J. Med. Vet. Mycol. 2: 357 – 367.
 

Opportunisten und andere Pilze - Diagnostik

Rüchel, R., M. Behe, B. Torp, and H. Laatsch. 2001. Usefulness of optical brighteners in medical mycology. Rev. Iberoam. Micol. 18: 147-149.

Weig, M., H. Klinker, B. H. Bögner, A. Meier, and U. Groß. 1997. Usefulness of Pneumocystis carinii PCR for PcP diagnosis in different patient groups. J. Clin. Microbiol. 35: 1445-1449.

Weig, M., H. Klinker, M. Wilhelm, K. Lemmer, and U. Groß. 1996. Correlation of Pneumocystis carinii-PCR with clinical diagnosis in immunocompromised patients. Lancet 347: 1266.
 

Opportunisten und andere Pilze - Klinik und Therapie

Binder, C., and R. Rüchel. 2000. Case Report. Mixed systemic mycosis with fatal outcome in a patient with acute myeloblastic leukaemia. Mycoses 43: 59-63

Ponton, J., R. Rüchel, K. V. Clemons et al. 2000. Emerging pathogens. Med. Mycol.  38, Suppl.1: 225-236

Wulf, G. G., U. Reichard, B. Wörmann, W. Hiddemann. 1998. Pneumocystis carinii pneumonia with peripheral nodular infiltrates in a patient with T-acute lymphoblastic leukemia. J. Clin. Oncol. 16: 3476-3477

Borg-von Zepelin, M., H. Eiffert, M. Kann, and R. Rüchel. 1993. Changes in the spectrum of fungal isolates: results from clinical specimens gathered in 1987/88 compared with those in 1991/92 in the University Hospital Göttingen, Germany. Mycoses 36: 247-253.
 

Opportunisten und andere Pilze -Forschung

Schoen, C., U. Reichard, M. Monod, H.D. Kratzin, and R. Rüchel. 2002. Molecular cloning of an extracellular aspartic proteinase from Rhizopus microsporus and evidence for ist expression during infection. Med. Mycol. 40: 61-71.

Bohne, W., D. J. P. Ferguson, K. Kohler, and U. Groß. 2000. Developmental expression of a tandemly repeated, glycine- and serine-rich spore wall protein in the microsporidian pathogen Encephalitozoon cuniculi. Infect. Immun. 68: 2268-2275.

Reichard, U., C. Y. Hung, P. W. Thomas, and G. T. Cole .2000. Disruption of the gene which encodes a serodiagnostic antigen and chitinase of the human fungal pathogen Coccidioides immitis. Infect. Immun. 68: 5830-5838
 

Übersichten

Monod, M., K. Jaton-Ogay, and U. Reichard. 1999. Aspergillus fumigatus secreted proteases as antigenic molecules and virulence factors; in: Aspergillus fumigatus. Biology, Clinical Aspects and Molecular Approaches to Pathogenicity; Hrsg. v. Brakhage, A. , B. Jahn, and A. Schmidt; 2; Karger AG, Basel, pp. 182-192

Rüchel, R. and U. Reichard. 1999. Pathogenesis and clinical presentation of aspergillosis; in: Aspergillus fumigatus. Biology, Clinical Aspects and Molecular Approaches to Pathogenicity; Hrsg. v. Brakhage, A., B. Jahn, and A. Schmidt; 2; Karger AG, Basel, pp. 21-43

Rüchel, R. 1999. Proteases of  pathogenic fungi. Mycoses 42: 48-52

Petry, F., and U. Groß. 1998. Kryptosporidiose und Mikrosporidiose bei Immunschwäche. Fortschr. Med. 116: 29-31 und Nr. III/1998: 71-75.

Reichard, U. 1998. The significance of secretory and structure-associated proteases of Aspergillus fumigatus for the pathogenesis of invasive aspergillosis. Mycoses 41: 78-82

Rüchel, R. 1998. Diagnostik von tiefen Mykosen. In: Mykosen in der operativen Medizin. Grundlagen der Diagnostik und Therapie. P. Kujath, Hrsg. Georg Thieme, Stuttgart, pp. 17-23

Hube, B., R. Rüchel, M. Monod, D. Sanglard, and F. C. Odds. 1998. Functional Aspects of Secreted Candida Proteinases Aspartic Proteinases, M. James, ed., Plenum Press, N.Y., pp.339-344

Weig, M., U. Groß, and F. Mühlschlegel. 1998. Clinical aspects and pathogenesis of Candida infection. Trends Microbiol. 6: 468-470.

Groß, U., M. Weig, and F. Petry. 1997. Opportunistische parasitologische Infektionen bei immunsupprimierten Patienten. Med. Welt 48: 158-168.

Groß, U., P.-M. Rath, and R. Rüchel. 1997. Molekulare und immunologische Untersuchungen bei Eukaryonten. Hyg. Mikrobiol. 2/97: 9-11.

Rüchel, R. 1995.Diagnostik der Candidamykosen. Internist 36: 125-127